Journal: Molecular Biology of the Cell

Article Title: Role of JAM-A tyrosine phosphorylation in epithelial barrier dysfunction during intestinal inflammation

doi: 10.1091/mbc.e18-08-0531

Figure Lengend Snippet: Figure 1 JAM-A is tyrosine phosphorylated at Y280. a: Human JAM-A highlighting

Article Snippet: Rabbit anti-p-JAM-A Y280 was from Rockland Inc., Limerick, PA, catalog: 600-401-GN5.

Techniques:

Journal: Molecular Biology of the Cell

Article Title: Role of JAM-A tyrosine phosphorylation in epithelial barrier dysfunction during intestinal inflammation

doi: 10.1091/mbc.e18-08-0531

Figure Lengend Snippet: Figure 3 Yes-1 regulates phosphorylation of JAM-A Y280. a: The Src family kinase inhibitor PP2 (1 M) inhibits pervanadate-dependent phosphorylation of JAM-A Y280. Immunofluorescence staining of p-JAM-A Y280 and total JAM-A in T84 monolayers pre- treated with/without PP2 followed by exposure to pervanadate. Similar results were observed in SK CO-15 cells (not shown). b: Dose-dependent inhibition of p-JAM-A Y280 by PP2 after pervanadate treatment. T84 cells were lysed after treatment with increasing concentrations of PP2 followed by exposure to pervanadate. Lysates were then immunoblotted for p-JAM-A Y280, total JAM-A, and total Src. P-EGFR Y1068 was used as a positive control for PP2. Calnexin was used as a loading control. c: Co- immunoprecipitation of Yes1/Lyn with JAM-A. SK CO-15 cells stably expressing HA- tagged full-length JAM-A were lysed with 1% Triton X-100 lysis buffer followed by

Article Snippet: Rabbit anti-p-JAM-A Y280 was from Rockland Inc., Limerick, PA, catalog: 600-401-GN5.

Techniques: Phospho-proteomics, Immunofluorescence, Staining, Inhibition, Positive Control, Control, Immunoprecipitation, Stable Transfection, Expressing, Lysis

Journal: Molecular Biology of the Cell

Article Title: Role of JAM-A tyrosine phosphorylation in epithelial barrier dysfunction during intestinal inflammation

doi: 10.1091/mbc.e18-08-0531

Figure Lengend Snippet: Figure 4. PTPN13 acts as a phosphatase for p-JAM-A Y280.

Article Snippet: Rabbit anti-p-JAM-A Y280 was from Rockland Inc., Limerick, PA, catalog: 600-401-GN5.

Techniques:

Journal: Molecular Biology of the Cell

Article Title: Role of JAM-A tyrosine phosphorylation in epithelial barrier dysfunction during intestinal inflammation

doi: 10.1091/mbc.e18-08-0531

Figure Lengend Snippet: Figure 5. Phosphorylation of JAM-A Y280 results in decreased JAM-A association

Article Snippet: Rabbit anti-p-JAM-A Y280 was from Rockland Inc., Limerick, PA, catalog: 600-401-GN5.

Techniques: Phospho-proteomics

Journal: Molecular Biology of the Cell

Article Title: Role of JAM-A tyrosine phosphorylation in epithelial barrier dysfunction during intestinal inflammation

doi: 10.1091/mbc.e18-08-0531

Figure Lengend Snippet: Figure 6 p-JAM-A Y280 is increased in mouse models of experimental intestinal

Article Snippet: Rabbit anti-p-JAM-A Y280 was from Rockland Inc., Limerick, PA, catalog: 600-401-GN5.

Techniques: